[期刊论文][Full-length article]


Interaction between sorghum procyanidin tetramers and the catalytic region of glucosyltransferases-I from Streptococcus mutans UA159

作   者:
Jianan Yu;Fangfang Yan;Qun Lu;Rui Liu;

出版年:2018

D  O  I:10.1016/j.foodres.2018.06.027

页     码:152 - 159
出版社:Elsevier BV


摘   要:

Sorghum procyanidins (SPC) tetramers were reported to have inhibitory effects on the adhesion of Streptococcus mutans (S. mutans), which is one of the primary pathogenic bacteria that cause caries. To make clear the mechanism underlying the inhibitory effects of SPC tetramers on the adhesion of S. mutans, it is important to understand the interaction between SPC tetramers and the catalytic region of glucosyltransferases-I (GTF-I/CAT) from S. mutans. In this study, fluorescence quenching, UV-visible (UV-Vis) absorption and circular dichroism (CD) spectroscopies were applied to investigate the interaction between SPC tetramers and the GTF-I/CAT from S. mutans UA159. The fluorescence quenching results demonstrated that SPC tetramers combined with GTF-I/CAT protein on one binding site, and the fluorescence intensity of GTF-I/CAT protein was decreased regularly by static quenching with increasing concentrations of SPC tetramers. The UV-Vis absorption spectra of GTF-I/CAT protein were also changed with a red shift owing to the impact of SPC tetramers. In addition, the proportions of α-helix, β-sheet and random coil in the secondary structure of GTF-I/CAT protein were obviously altered by SPC tetramers. In conclusion, SPC tetramers have a strong affinity for GTF-I/CAT protein by altering its conformation and micro-environment. Our results suggest that SPC tetramers interact with GTF-I/CAT to interfere with the adhesion of S. mutans, which may facilitate the better application of SPC tetramers in the prevention of dental caries. Copyright © 2018 Elsevier Ltd. All rights reserved.



关键字:

Sorghum ; Procyanidins ; Glucosyltransferases-I ; Fluorescence quenching ; Circular dichroism


所属期刊
Food Research International
ISSN: 0963-9969
来自:Elsevier BV