[期刊论文]


Interaction with Deoxyribonucleic Acid and Determination of Orientin in Lophatherum gracile Brongn by High-Performance Liquid Chromatography with Amperometric Detection

作   者:
Fang Wang;Fangfang Yan;Yuling Long;Lili Wang;Zilin Chen;

出版年:2015

D  O  I:10.1016/j.electacta.2015.08.094

页     码:829 - 837
出版社:Elsevier BV


摘   要:

The mechanisms of the electrochemical oxidation of orientin and its interaction with deoxyribonucleic acid have been investigated using glassy carbon electrode. The electrochemical response of orientin is due to oxidation of phenolic hydroxyl groups on orientin. The whole process is controlled by the adsorption step and concerned 4 electrons and 4 protons. Negative shift of potential and decrease of peak current for electrochemical oxidation of orientin can be observed at bare glassy carbon electrode and deoxyribonucleic acid modified electrode in 0.05M Na"2HPO"4-KH"2PO"4 (pH 7.48), confirming the dominant electrostatic interaction between orientin and deoxyribonucleic acid. Moreover, a reliable and sensitive method of reversed-phase high-performance liquid chromatography-electrochemical detection has been developed for simultaneous determination of the isomer pair orientin/isoorientin. Chromatographic separation was carried out on a reversed-phase C"1"8 column and a mobile phase comprised of acetonitrile and acetate (0.5%, pH 2.97) by amperometric detection with a glassy carbon electrode at the working potential of +1.00V. The method was validated for linearity, accuracy, precision, and limit of detection. Under the optimized conditions, linear regression analysis for the calibration curve showed a good linear relationship between peak area and their concentrations in the linear range of 89.2nM to 59.5@mM for orientin with detection limit of 14.9nM and 78.0nM to 52.0@mM for isoorientin with a detection limit of 13.0nM, respectively. Compared to the method using ultraviolet detection, the detection limits are greatly lowered. Finally, the proposed method has been successfully applied to the determination of orientin and isoorientin in Lophatherum gracile Brongn.



关键字:

Orientin ; Deoxyribonucleic acid (DNA) ; High-performance liquid chromatography (HPLC) ; Amperometric detection ; Lophatherum gracile Brongn


所属期刊
Electrochimica Acta
ISSN: 0013-4686
来自:Elsevier BV