[期刊论文]


Matrine inhibits 3T3-L1 preadipocyte differentiation associated with suppression of ERK1/2 phosphorylation

作   者:
Yifan Xing;Fangfang Yan;Yan Liu;Yunfang Liu;Yuxia Zhao;

出版年:2010

D  O  I:10.1016/j.bbrc.2010.04.163

页     码:691 - 695
出版社:Elsevier BV


摘   要:

In this study, we examined whether matrine could inhibit the differentiation of 3T3-L1 preadipocytes and further explored the possible inhibitory mechanisms. Evidenced by Oil Red O staining and AdipoRed assay, matrine dose-dependently inhibited lipid accumulation at concentrations of 125, 250 and 500 microg/ml. At molecular level, the expression of transcription factors, PPARgamma and C/EBPalpha, was reduced by matrine during adipogenesis. After treatment for 6 days, the mRNA levels of adipocyte-specific genes, such as aP2, LPL, adiponectin and leptin, were also down-regulated by matrine in a dose-dependent manner. Moreover, 500 microg/ml matrine inhibited the phosphorylation of ERK1/2 at the early stage of differentiation. Our results indicate that inhibition of 3T3-L1 preadipocyte differentiation by matrine is associated with the suppression of ERK1/2 phosphorylation. Thus, matrine has the potential to be an alternative natural product for the treatment of obesity. Copyright (c) 2010 Elsevier Inc. All rights reserved.



关键字:

Matrine ; Adipocyte ; Differentiation ; p-ERK1/2


所属期刊
Biochemical and Biophysical Research Communications
ISSN: 0006-291X
来自:Elsevier BV